Performance of a single-photon laser-scanning confocal fluorescence microscope

Authors

Godofredo S. Bautista Jr. ⋅ PH National Institute of Physics, University of the Philippines Diliman
Jelda Jayne Miranda ⋅ PH National Institute of Physics, University of the Philippines Diliman
Vincent Ricardo Daria ⋅ PH National Institute of Physics, University of the Philippines Diliman
Caesar Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We investigated the performance of a single-photon laser-scanning confocal fluorescence microscope (SP-LSCFM) constructed by attaching a beam-scanning optical setup to a conventional optical microscope. The optical sectioning capability of the SP-LSCFM is demonstrated by imaging 10-μm diameter FITC beads and an Alexa Fluor stained star-shaped alga, using the 488-nm line of an argon-ion laser. Results show successful high-contrast lateral images corresponding to the achievement of axial discrimination.

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Issue

2002: Proceedings of the 20th Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2002-1D-01

Section

Instrumentation and Optics

Published

2002-10-23

How to Cite

[1]

GS Bautista, JJ Miranda, VR Daria, and C Saloma, Performance of a single-photon laser-scanning confocal fluorescence microscope, Proceedings of the Samahang Pisika ng Pilipinas 20, SPP-2002-1D-01 (2002). URL: https://proceedings.spp-online.org/article/view/SPP-2002-1D-01.