Performance of a single-photon laser-scanning confocal fluorescence microscope

Authors

  • Godofredo S. Bautista Jr. ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Jelda Jayne Miranda ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Vincent Ricardo Daria ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Caesar Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We investigated the performance of a single-photon laser-scanning confocal fluorescence microscope (SP-LSCFM) constructed by attaching a beam-scanning optical setup to a conventional optical microscope. The optical sectioning capability of the SP-LSCFM is demonstrated by imaging 10-μm diameter FITC beads and an Alexa Fluor stained star-shaped alga, using the 488-nm line of an argon-ion laser. Results show successful high-contrast lateral images corresponding to the achievement of axial discrimination.

SPP 2002 Proceedings Cover

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Published

2002-10-23

Issue

2002: Proceedings of the 20th Samahang Pisika ng Pilipinas Physics Congress

Section

Instrumentation and Optics

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How to Cite

[1]
“Performance of a single-photon laser-scanning confocal fluorescence microscope”, Proc. SPP, vol. 20, no. 1, pp. SPP–2002, Oct. 2002, Accessed: Apr. 08, 2026. [Online]. Available: https://proceedings.spp-online.org/article/view/SPP-2002-1D-01