Performance of a single-photon laser-scanning confocal fluorescence microscope

Authors

Godofredo S. Bautista Jr. National Institute of Physics, University of the Philippines Diliman
Jelda Jayne Miranda National Institute of Physics, University of the Philippines Diliman
Vincent Ricardo Daria National Institute of Physics, University of the Philippines Diliman
Caesar Saloma National Institute of Physics, University of the Philippines Diliman

Abstract

We investigated the performance of a single-photon laser-scanning confocal fluorescence microscope (SP-LSCFM) constructed by attaching a beam-scanning optical setup to a conventional optical microscope. The optical sectioning capability of the SP-LSCFM is demonstrated by imaging 10-μm diameter FITC beads and an Alexa Fluor stained star-shaped alga, using the 488-nm line of an argon-ion laser. Results show successful high-contrast lateral images corresponding to the achievement of axial discrimination.

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Issue

2002: Proceedings of the 20th Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2002-1D-01

Section

Instrumentation and Optics

Published

2002-10-23

How to Cite

[1]

GS Bautista, JJ Miranda, VR Daria, and C Saloma, Performance of a single-photon laser-scanning confocal fluorescence microscope, Proceedings of the Samahang Pisika ng Pilipinas 20, SPP-2002-1D-01 (2002). URL: https://proceedings.spp-online.org/article/view/SPP-2002-1D-01.