Influence on DNA cleavage activities by mutations in CRISPR/Cas9

Authors

Haibin Su ⋅ HK Department of Chemistry, Hong Kong University of Science and Technology

Abstract

In this work, a domain motion dependent rate model is constructed for a comprehensive understanding of CRISPR Cas9 dynamics. Differences in cleavage efficiencies and off-target rates are essentially due to altered interaction inside Cas9:RNA:DNA complex with mutated residues. We applied pair correlation analysis to whole Cas9 protein sequences to unravel structural organization of proteins. With the valuable 3D structures from experiments and simulations, the roles of mutations are clarified to shed light on bacteria-phage co-evolution strategies.

About the Speaker

Haibin Su, Department of Chemistry, Hong Kong University of Science and Technology

Dr. Haibin Su is an Associate Professor at Department of Chemistry in The Hong Kong University of Science and Technology since 2018. He graduated from University of Stony Brook, while performing his thesis projects in Center for Data Intensive Computing at Brookhaven National Laboratory. Then he went to Caltech for his PostDoc prior to joining Nanyang Technological University in 2005. His research interest includes development and application of theoretical and computational physical science: i.e., quantum-mechanical and classical simulations and modeling of the structural, kinetic, and dynamic properties of complex functional systems, in particular emergent collective properties at multiple spatial and temporal scales.

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Issue

2021: Proceedings of the 39th Samahang Pisika ng Pilipinas Physics Conference

Article ID

SPP-2021-INV-2G-01

Section

Invited Presentations

Published

2021-09-18

How to Cite

[1]

H Su, Influence on DNA cleavage activities by mutations in CRISPR/Cas9, Proceedings of the Samahang Pisika ng Pilipinas 39, SPP-2021-INV-2G-01 (2021). URL: https://proceedings.spp-online.org/article/view/SPP-2021-INV-2G-01.