Influence on DNA cleavage activities by mutations in CRISPR/Cas9
In this work, a domain motion dependent rate model is constructed for a comprehensive understanding of CRISPR Cas9 dynamics. Differences in cleavage efficiencies and off-target rates are essentially due to altered interaction inside Cas9:RNA:DNA complex with mutated residues. We applied pair correlation analysis to whole Cas9 protein sequences to unravel structural organization of proteins. With the valuable 3D structures from experiments and simulations, the roles of mutations are clarified to shed light on bacteria-phage co-evolution strategies.