Viscoelasticity of dilute DNA solutions using video microscopy

Authors

Florencio S. Bustamante ⋅ PH Medical Biophysics Group, Department of Physics, University of San Carlos
Amos M. Santoya ⋅ PH Medical Biophysics Group, Department of Physics, University of San Carlos
John Philip T. Billones ⋅ PH Medical Biophysics Group, Department of Physics, University of San Carlos
John Paul Geraldino ⋅ PH Department of Physics, University of San Carlos
Rommel G. Bacabac ⋅ PH Department of Physics, University of San Carlos

Abstract

We investigated the viscoelastic properties of dilute solutions of whole genomic DNA by quantifying the complex shear modulus for varying DNA concentrations. It was observed that both the elastic and viscous moduli were directly proportional to concentration. The crossover frequency increases with the DNA concentration, which implies that the liquid-like property of the concentrated DNA solution will only dominate at much higher frequencies since it stores more energy and in consequence has a relatively short relaxation time. These findings enable us to fine-tune our DNA network as a model for other biopolymer networks by simply adjusting the concentration.

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Issue

2013: Proceedings of the 31st Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP2013-2B-6

Section

Optics and Signal Processing

Published

2013-10-23

How to Cite

[1]

FS Bustamante, AM Santoya, JPT Billones, JP Geraldino, and RG Bacabac, Viscoelasticity of dilute DNA solutions using video microscopy, Proceedings of the Samahang Pisika ng Pilipinas 31, SPP2013-2B-6 (2013). URL: https://proceedings.spp-online.org/article/view/SPP2013-2B-6.