Simultaneous application of constraints in filter-based fluorescence spectral microscopy

Authors

Luis Buño ⋅ PH National Institute of Physics, University of the Philippines Diliman
Giovanni Tapang ⋅ PH National Institute of Physics, University of the Philippines Diliman
Cynthia Palmes-Saloma ⋅ PH National Institute of Molecular Biology and Biotechnology, University of the Philippines, Diliman
Caesar Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We demonstrate the simultaneous application of the minimum negativity and finite support constraints on primary spectra that are obtained via filter-based fluorescence spectral microscopy. Conservation of energy constraint is then applied on the recovered spectrum to improve final reconstruction quality. Simultaneous application improves the computational efficiency of the spectral recovery algorithm and enhances the reconstruction quality when the raw signal is above a critical fraction of the theoretical maximum of intensity values. Dependence of the spectral reconstruction on the emission wavelength of the fluorescent probe is also analyzed. Spectral recovery is demonstrated

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Issue

2011: Proceedings of the 29th Samahang Pisika ng Pilipinas National Physics Congress

Article ID

SPP2011-4B-1

Section

Acoustics, Optics, Photonics

Published

2019-02-17

How to Cite

[1]

L Buño, G Tapang, C Palmes-Saloma, and C Saloma, Simultaneous application of constraints in filter-based fluorescence spectral microscopy, Proceedings of the Samahang Pisika ng Pilipinas 29, SPP2011-4B-1 (2019). URL: https://proceedings.spp-online.org/article/view/SPP2011-4B-1.