Rheological characterization of fibrin-carrageenan composite hydrogels for 3D extrusion printing

Authors

Cindy L. Flores ⋅ PH Department of Physics, University of San Carlos, Cebu City
Mary Diane A. Pilapil ⋅ PH Department of Physics, University of San Carlos, Cebu City
Zachary J. Borromeo ⋅ PH Department of Physics, University of San Carlos, Cebu City
Mark Adones P. Lingaro ⋅ PH Department of Physics, University of San Carlos, Cebu City
Grezeil B. Edar ⋅ PH Department of Physics, University of San Carlos, Cebu City
Lourenz O. Baliber ⋅ PH Department of Physics, University of San Carlos, Cebu City
Hyacinth N. Suarez ⋅ PH Holy Name University, Tagbilaran City
Rommel G. Bacabac ⋅ PH Department of Physics, University of San Carlos, Cebu City

Abstract

Fibrin-carrageenan composite hydrogels (FCCHs) are novel biomaterials with potential applications as artificial muscle tissue scaffolds. Fibrin is a crucial component for blood clotting and wound healing, and carrageenan is known to promote cell growth and differentiation. Both fibrin and carrageenan's mechanical properties allow for flexibility and tunability of the mixtures' viscoelasticity. This study investigated these novel hydrogels' rheology, printability, and biocompatibility. The FCCHs were assessed for stability, frequency dependence, and strain response. Furthermore, the strand width and pore printability were also compared with the control commercial LAMININK samples from CELLINK. Finally, in vitro cytotoxicity of the samples was tested using the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay with NIH/3T3 fibroblasts. Results showed that the FCCH behaved similarly to that of the commercial ink in both its viscoelastic and printability properties. These results suggest that FCCH is a viable biomaterial ink for 3D-printed artificial muscle scaffolds.

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Issue

2024: Proceedings of the 42nd Samahang Pisika ng Pilipinas Physics Conference

Article ID

SPP-2024-PA-21

Section

Poster Session A (Condensed Matter Physics and Materials Science)

Published

2024-06-28

How to Cite

[1]

CL Flores, MDA Pilapil, ZJ Borromeo, MAP Lingaro, GB Edar, LO Baliber, HN Suarez, and RG Bacabac, Rheological characterization of fibrin-carrageenan composite hydrogels for 3D extrusion printing, Proceedings of the Samahang Pisika ng Pilipinas 42, SPP-2024-PA-21 (2024). URL: https://proceedings.spp-online.org/article/view/SPP-2024-PA-21.