Rheological Characterization of Fibrin-Carrageenan Composite Hydrogels for 3D Extrusion Printing

Authors

Cindy Flores Department of Physics, University of San Carlos, Nasipit, Talamban, Cebu City, Philippines
Mary Diane Arcenal Pilapil University of San Carlos
Zachary Justiniane Borromeo Department of Physics, University of San Carlos, Nasipit, Talamban, Cebu City, Philippines
Mark Adones Puaben Lingaro Department of Physics, University of San Carlos, Nasipit, Talamban, Cebu City, Philippines
Grezeil Edar Department of Physics, University of San Carlos, Nasipit, Talamban, Cebu City, Philippines
Lourenz Baliber Department of Physics, University of San Carlos, Nasipit, Talamban, Cebu City, Philippines
Hyacinth Suarez Holy Name University, Dampas District, Tagbilaran City, Philippines
Rommel Bacabac Department of Physics, University of San Carlos, Nasipit, Talamban, Cebu City, Philippines

Abstract

Fibrin-carrageenan composite hydrogels (FCCHs) are novel biomaterials with potential applications as artificial muscle tissue scaffolds. Fibrin is a crucial component for blood clotting and wound healing, and carrageenan is known to promote cell growth and differentiation. Both fibrin and carrageenan’s mechanical properties allow for flexibility and tunability of the mixtures’ viscoelasticity. This study investigated these novel hydrogels’ rheology, printability, and biocompatibility. The FCCHs were assessed for stability, frequency dependence, and strain response. Furthermore, the strand width and pore printability were also compared with the control commercial LAMININK samples from CELLINK. Finally, in vitro cytotoxicity of the samples was tested using the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay with NIH/3T3 fibroblasts. Results showed that the FCCH behaved similarly to that of the commercial ink in both its viscoelastic and printability properties. These results suggest that FCCH is a viable biomaterial ink for 3D-printed artificial muscle scaffolds

Issue

2024: Proceedings of the 42nd Samahang Pisika ng Pilipinas Physics Conference

Article ID

SPP-2024-PA-21

Section

Poster Session A (Condensed Matter Physics and Materials Science)

Published

2024-06-28

How to Cite

[1]

C Flores, MDA Pilapil, ZJ Borromeo, MAP Lingaro, G Edar, L Baliber, H Suarez, and R Bacabac, Rheological Characterization of Fibrin-Carrageenan Composite Hydrogels for 3D Extrusion Printing, Proceedings of the Samahang Pisika ng Pilipinas 42, SPP-2024-PA-21 (2024). URL: https://proceedings.spp-online.org/article/view/SPP-2024-PA-21.