Passive microrheology in carrageenan-fibrin mixtures using video microscopy

Authors

  • Lourenz Baliber ⋅ PH Department of Physics, University of San Carlos, Cebu City
  • Zachary Borromeo ⋅ PH Department of Physics, University of San Carlos, Cebu City
  • Mark Adones Lingaro ⋅ PH Department of Physics, University of San Carlos, Cebu City
  • Mary Diane Pilapil ⋅ PH Department of Physics, University of San Carlos, Cebu City
  • Grezeil Edar ⋅ PH Department of Physics, University of San Carlos, Cebu City
  • Cindy Flores ⋅ PH Department of Physics, University of San Carlos, Cebu City
  • Hyacinth Suarez ⋅ PH Holy Name University, Dampas District, Tagbilaran City
  • Rommel Bacabac ⋅ PH Department of Physics, University of San Carlos, Cebu City

Abstract

Passive microrheology was demonstrated to assess the viscoelastic properties of carrageenan-fibrin mixtures. Carrageenan-based hydrogels have become promising candidates as biomimetic materials due to their biocompatible and non-cytotoxic properties. The colloidal probe particles were freely-diffusing in the aforementioned mixture, with their movement traced by video microscopy. Their motion was quantified using mean square displacement (MSD), obtained from the generalizations of the Stokes-Einstein relations. The analysis of tracer particle movement revealed that at high fibrin concentrations, the particles were eventually confined and exhibited sub-diffusion behavior based on their MSD in contrast to pure samples. Moreover, the G' and G'' of the carrageenan-fibrin mixtures calculated from the unilateral Laplace transform of the MSD revealed the changes in the material's viscoelastic behavior at varying concentrations. Although, systematic errors may arise from the microscope-camera setup calibration including random noise and drifting of tracer particles. This study shows a new and alternative way to probe into the microrheological properties of carrageenan-fibrin mixtures using video microscopy involving the implementation of particle tracking. Thereby elucidating the correlation of the tracer particle's MSD and the biopolymer's viscoelastic properties.

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Issue

Brewing waves of innovation and discovery in Physics
3-6 July 2024, Batangas State University, Pablo Borbon Campus

Please visit the SPP2024 activity webpage for more information on this year's Physics Congress.

Article ID

SPP-2024-1E-05

Section

Complex Systems and Data Analytics

Published

2024-06-30

How to Cite

[1]
L Baliber, Z Borromeo, MA Lingaro, MD Pilapil, G Edar, C Flores, H Suarez, and R Bacabac, Passive microrheology in carrageenan-fibrin mixtures using video microscopy, Proceedings of the Samahang Pisika ng Pilipinas 42, SPP-2024-1E-05 (2024). URL: https://proceedings.spp-online.org/article/view/SPP-2024-1E-05.