All optical detection of T4 bacteriophage via near-infrared transmission spectroscopy
The T4 bacteriophage is a bacteriophage species that infects Escherichia coli bacteria. The T4 bacteriophage has similar dimensions to the SARS-CoV-2 virus and may be used as a medically safe alternative for performing initial coronavirus detection techniques without the necessity for level 2 biohazard safety precautions. A proof-of-concept demonstration of the detection of T4 bacteriophage suspended in phage buffer 150 mM NaCl, 40 mM Tris-HCl (pH 7.4), and 10 mM MgSO4, with double distilled water was performed using near-infrared transmission spectroscopy. A characteristic transmission dip occurring at 6800 Å was observed for T4 bacteriophage concentration comparable to the SARS-CoV-2 viral load in the saliva of an infected human.