All optical detection of T4 bacteriophage via near-infrared transmission spectroscopy

Authors

Ivan Cedrick M. Verona ⋅ PH National Institute of Physics, University of the Philippines Diliman
Grace Lachica ⋅ PH Philippine Genome Center–Program for Agriculture, University of the Philippines Los Baños
Vince Paul Juguilon ⋅ PH National Institute of Physics, University of the Philippines Diliman
Alexander De Los Reyes ⋅ PH National Institute of Physics, University of the Philippines Diliman
Hannah Bardolaza ⋅ PH Materials Science and Engineering Program, University of the Philippines Diliman
Neil Irvin Cabello ⋅ PH National Institute of Physics, University of the Philippines Diliman
Gonzalo Serafica ⋅ PH Corresponding Member, National Academy of Science and Technology
Elmer Estacio ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

The T4 bacteriophage is a bacteriophage species that infects Escherichia coli bacteria. The T4 bacteriophage has similar dimensions to the SARS-CoV-2 virus and may be used as a medically safe alternative for performing initial coronavirus detection techniques without the necessity for level 2 biohazard safety precautions. A proof-of-concept demonstration of the detection of T4 bacteriophage suspended in phage buffer 150 mM NaCl, 40 mM Tris-HCl (pH 7.4), and 10 mM MgSO₄, with double distilled water was performed using near-infrared transmission spectroscopy. A characteristic transmission dip occurring at 6800 Å was observed for T4 bacteriophage concentration comparable to the SARS-CoV-2 viral load in the saliva of an infected human.

Downloads

Issue

2021: Proceedings of the 39th Samahang Pisika ng Pilipinas Physics Conference

Article ID

SPP-2021-2B-05

Section

Atomic, Molecular, and Optical Physics

Published

2021-10-14

How to Cite

[1]

ICM Verona, G Lachica, VP Juguilon, A De Los Reyes, H Bardolaza, NI Cabello, G Serafica, and E Estacio, All optical detection of T4 bacteriophage via near-infrared transmission spectroscopy, Proceedings of the Samahang Pisika ng Pilipinas 39, SPP-2021-2B-05 (2021). URL: https://proceedings.spp-online.org/article/view/SPP-2021-2B-05.