All optical detection of T4 bacteriophage via near-infrared transmission spectroscopy

Authors

Ivan Cedrick M. Verona National Institute of Physics, University of the Philippines Diliman
Grace Lachica Philippine Genome Center–Program for Agriculture, University of the Philippines Los Baños
Vince Paul Juguilon National Institute of Physics, University of the Philippines Diliman
Alexander De Los Reyes National Institute of Physics, University of the Philippines Diliman
Hannah Bardolaza Materials Science and Engineering Program, University of the Philippines Diliman
Neil Irvin Cabello National Institute of Physics, University of the Philippines Diliman
Gonzalo Serafica Corresponding Member, National Academy of Science and Technology
Elmer Estacio National Institute of Physics, University of the Philippines Diliman

Abstract

The T4 bacteriophage is a bacteriophage species that infects Escherichia coli bacteria. The T4 bacteriophage has similar dimensions to the SARS-CoV-2 virus and may be used as a medically safe alternative for performing initial coronavirus detection techniques without the necessity for level 2 biohazard safety precautions. A proof-of-concept demonstration of the detection of T4 bacteriophage suspended in phage buffer 150 mM NaCl, 40 mM Tris-HCl (pH 7.4), and 10 mM MgSO₄, with double distilled water was performed using near-infrared transmission spectroscopy. A characteristic transmission dip occurring at 6800 Å was observed for T4 bacteriophage concentration comparable to the SARS-CoV-2 viral load in the saliva of an infected human.

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Issue

2021: Proceedings of the 39th Samahang Pisika ng Pilipinas Physics Conference

Article ID

SPP-2021-2B-05

Section

Atomic, Molecular, and Optical Physics

Published

2021-10-14

How to Cite

[1]

ICM Verona, G Lachica, VP Juguilon, A De Los Reyes, H Bardolaza, NI Cabello, G Serafica, and E Estacio, All optical detection of T4 bacteriophage via near-infrared transmission spectroscopy, Proceedings of the Samahang Pisika ng Pilipinas 39, SPP-2021-2B-05 (2021). URL: https://proceedings.spp-online.org/article/view/SPP-2021-2B-05.