Fluorescence suppression and surface-enhanced Raman scattering (SERS) in rhodamine B dye using silver nanowires as SERS substrates

Authors

Horace Andrew Fernandez Husay ⋅ PH National Institute of Physics, University of the Philippines Diliman
Jeremias Ibus-Armonia ⋅ PH National Institute of Physics, University of the Philippines Diliman
Laureen Ida Monzon Ballesteros ⋅ PH Materials Science and Engineering Program, University of the Philippines Diliman
Debinya Banisal Buenafe ⋅ PH Materials Science and Engineering Program, University of the Philippines Diliman
Ferdinand Tamayo Cayabyab, Jr ⋅ PH National Institute of Physics, University of the Philippines Diliman
Nathaniel T De Guzman ⋅ PH Department of Mining, Metallurgical, and Materials Engineering, University of the Philippines Diliman
Mary Donnabelle L Balela ⋅ PH Department of Mining, Metallurgical, and Materials Engineering, University of the Philippines Diliman
Elmer Surat Estacio ⋅ PH National Institute of Physics, University of the Philippines Diliman
Arnel Angud Salvador ⋅ PH National Institute of Physics, University of the Philippines Diliman
Armando Soriano Somintac ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We investigated the fluorescence suppression and surface-enhanced Raman scattering (SERS) in rhodamine B dye using silver nanowires (Ag NWs) as SERS substrates. The Ag NWs were prepared by electroless deposition and subsequently suspended in ethanol. The NWs were deposited on paper and used to detect rhodamine B (RhB) in water to a concentration of 5 µM. Fluorescence and Raman measurements were performed using a micro-Raman spectroscopy setup with a 532 nm excitation. Optical microscope images showed the presence of fluorescent regions where only a few NWs were observed and dark regions where a dense amount of Ag NWs were located. The fluorescence emission centered at ~581 nm was attributed to the RhB dye. A ~75% decrease in the fluorescence emission was observed at the SERS hotspots with Ag NWs. Raman measurements performed at the Ag NW-filled region confirmed that the vibrational modes of RhB were enhanced by a factor of 10⁴. This study demonstrates the feasibility of using Ag NWs to suppress fluorescence in biological or inorganic samples for Raman microscopy.

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Issue

2018: Proceedings of the 36th Samahang Pisika ng Pilipinas Physics Conference

Article ID

SPP-2018-PC-52

Section

Poster Session C (Mathematical Physics, Optics, and Interdisciplinary Topics)

Published

2018-06-01

How to Cite

[1]

HAF Husay, J Ibus-Armonia, LIM Ballesteros, DB Buenafe, FT Cayabyab, NT De Guzman, MDL Balela, ES Estacio, AA Salvador, and AS Somintac, Fluorescence suppression and surface-enhanced Raman scattering (SERS) in rhodamine B dye using silver nanowires as SERS substrates, Proceedings of the Samahang Pisika ng Pilipinas 36, SPP-2018-PC-52 (2018). URL: https://proceedings.spp-online.org/article/view/SPP-2018-PC-52.