Three-dimensional light fields for neurophotonics and micromanipulation

Authors

Vincent R. Daria ⋅ AU Research School of Physics and Engineering and John Curtin School of Medical Research, The Australian National University
Mary Ann Go ⋅ AU John Curtin School of Medical Research, The Australian National University
Christian Stricker ⋅ AU John Curtin School of Medical Research, The Australian National University
Steve Redman ⋅ AU John Curtin School of Medical Research, The Australian National University
Hans Bachor ⋅ AU Research School of Physics and Engineering, The Australian National University

Abstract

We describe a multi-functional system that projects three-dimensional (3D) light fields for experiments in neurophotonics and for multi-beam optical tweezers. Here, we use a single laser to project 3D light fields via holographic projection. When the laser is operated in femtosecond pulse mode, we show that the projected 3D light patterns have sufficient spatiotemporal photon density for multi-site two-photon excitation of biological fluorescent markers. We show that these light patterns can be used for photolysis of caged neurotransmitters for analyzing the functionality and connectivity of neuronal networks. When the same laser operates in continuous-wave mode, we can use the system for multi-beam optical tweezers to manipulate an array of particles simultaneously.

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Issue

2010: Proceedings of the 28th Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2010-PS-08

Section

Plenary Sessions

Published

2010-10-25

How to Cite

[1]

VR Daria, MA Go, C Stricker, S Redman, and H Bachor, Three-dimensional light fields for neurophotonics and micromanipulation, Proceedings of the Samahang Pisika ng Pilipinas 28, SPP-2010-PS-08 (2010). URL: https://proceedings.spp-online.org/article/view/SPP-2010-PS-08.