Three-dimensional light fields for neurophotonics and micromanipulation

Authors

  • Vincent R. Daria Research School of Physics and Engineering and John Curtin School of Medical Research, The Australian National University
  • Mary Ann Go John Curtin School of Medical Research, The Australian National University
  • Christian Stricker John Curtin School of Medical Research, The Australian National University
  • Steve Redman John Curtin School of Medical Research, The Australian National University
  • Hans Bachor Research School of Physics and Engineering, The Australian National University

Abstract

We describe a multi-functional system that projects three-dimensional (3D) light fields for experiments in neurophotonics and for multi-beam optical tweezers. Here, we use a single laser to project 3D light fields via holographic projection. When the laser is operated in femtosecond pulse mode, we show that the projected 3D light patterns have sufficient spatiotemporal photon density for multi-site two-photon excitation of biological fluorescent markers. We show that these light patterns can be used for photolysis of caged neurotransmitters for analyzing the functionality and connectivity of neuronal networks. When the same laser operates in continuous-wave mode, we can use the system for multi-beam optical tweezers to manipulate an array of particles simultaneously.

SPP 2010 Proceedings Cover

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Published

2010-10-25

Issue

2010: Proceedings of the 28th Samahang Pisika ng Pilipinas Physics Congress

Section

Plenary Sessions

License

By submitting their manuscript to the Samahang Pisika ng Pilipinas (SPP) for consideration, the Authors warrant that their work is original, does not infringe on existing copyrights, and is not under active consideration for publication elsewhere.
Upon acceptance of their manuscript, the Authors further agree to grant SPP the non-exclusive, worldwide, and royalty-free rights to record, edit, copy, reproduce, publish, distribute, and use all or part of the manuscript for any purpose, in any media now existing or developed in the future, either individually or as part of a collection.
All other associated economic and moral rights as granted by the Intellectual Property Code of the Philippines are maintained by the Authors.

How to Cite

[1]
“Three-dimensional light fields for neurophotonics and micromanipulation”, Proc. SPP, vol. 28, no. 1, p. SPP-2010-PS-08, Oct. 2010, Accessed: Mar. 26, 2026. [Online]. Available: https://proceedings.spp-online.org/article/view/SPP-2010-PS-08