Fluorescence experiments with dual-state chlorophyll immobilized by silicone

Abstract

The distinct effect of Sylgard-184 on the spectral characteristics of chlorophyll is presented. Spectral data of different concentrations of chlorophyll and chlorophyll adsorbed elastomer excited by a laser were collected. Emission spectra of two states of chlorophyll are remarkably different. The red shift that occurred in the chlorophyll-doped elastomer is smaller by 4.0 nm compared to chlorophyll in solution. When chlorophyll stock solution was sprayed on glass and polystyrene, the fluorescence peak wavelength of the chlorophyll-adsorbed elastomer is lower than the two other hosts by 8.0 nm. These results show that immobilization of chlorophyll in Sylgard-184 presents the possibility of new optical sensing material.