Laser scanning two-photon fluorescence microscope with a virtual pinhole

Authors

  • Paul Hilario ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Godofredo Bautista ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Vincent Daria ⋅ PH National Institute of Physics, University of the Philippines Diliman and Physics Department, Australian National University

Abstract

We construct and evaluate the performance of a two-photon fluorescence (2PF) microscope with a virtual pinhole. Instead of using a single-channel detector to render a 2PF image, we detect a wide-field image using multi channel pixelated array detector. As proof-of-principle, we use a conventional digital CCD camera to function as the wide-field detector. The virtual pinhole is implemented by selecting a region of interest in the wide field digital image and setting the pixel values outside the region to zero. The virtual pinhole removes scattered signals resulting to improve contrast and the visibility of details masked by scattered signals. The optical sectioning capability of the system was also shown.

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Issue

Article ID

SPP-2008-PA-28

Section

Poster Session A (Materials, Optical, and Plasma Physics)

Published

2008-10-22

How to Cite

[1]
P Hilario, G Bautista, and V Daria, Laser scanning two-photon fluorescence microscope with a virtual pinhole, Proceedings of the Samahang Pisika ng Pilipinas 26, SPP-2008-PA-28 (2008). URL: https://proceedings.spp-online.org/article/view/SPP-2008-PA-28.