A binary quenching mechanism for optical fluorescence sensing

Abstract

A binary, one-dimensional phenomenology of fluorescence quenching according to Lackowicz is presented. This phenomenology is shown to satisfactorily describe the quenching of Rhodamine 6G and Quinine by dextrose and salt. Maximum amounts of 8.2 mM of dextrose and salt were resolved, suggesting potential medical applications of fluorescence quenching. The dependence of this description of fluorescence quenching on the geometry of the fluorophores and quenchers is highlighted.