Controlled cell growth using holographic optical traps

Authors

Godofredo Bautista ⋅ PH National Institute of Physics, University of the Philippines Diliman
Reniel Cabral ⋅ PH National Institute of Physics, University of the Philippines Diliman
Mary Grace Bato ⋅ PH National Institute of Physics, University of the Philippines Diliman
Vincent Daria ⋅ PH National Institute of Physics, University of the Philippines Diliman and Physics Department, Australian National University

Abstract

We demonstrate controlled growth of yeast cells using holographic optical traps generated by encoding a computerdesigned hologram on a phase-only spatial light modulator. A Ti:S femtosecond laser operating at a wavelength of 790 nm under continuous-wave (CW) mode is used as the trapping source. We show simultaneous trapping of three growing yeast cells that are initially beside each other under a controlled environment. This protocol benchmarks a tool for initially studying the growth relationships between confined cells in mixed cultures.

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Issue

2008: Proceedings of the 26th Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2008-4D-03

Section

Instrumentation Physics

Published

2008-10-22

How to Cite

[1]

G Bautista, R Cabral, MG Bato, and V Daria, Controlled cell growth using holographic optical traps, Proceedings of the Samahang Pisika ng Pilipinas 26, SPP-2008-4D-03 (2008). URL: https://proceedings.spp-online.org/article/view/SPP-2008-4D-03.