Two dimensional concentration maps through spectral estimation and spectral unmixing
Abstract
We developed a spectral imaging microscope that relies only on emission signals with no additional optics added to the microscope. Thus, it can resolve relative concentration in samples with dimensions below the diffraction limit and there is no need to see the actual structure of a sample to determine its composition as long as it emits light. From image colour we estimate the spectral emission of the sample. From the estimated fluorescence emission of multiple overlapping microscopic species, we demonstrate the microscope's spectral unmixing performance on light emitting diodes (LEDs), co-localized multi-color single-stained fluorescent microspheres, and multistained chromosomes and generate their two dimensional concentration maps.
Downloads
Issue
Physics: Key to understanding nature
Liknayan: Susi sa pagtuklas ng kalikasan
24-26 October 2007, University of the Philippines Los BaƱos, Laguna