Two dimensional concentration maps through spectral estimation and spectral unmixing

Authors

  • Wilma R. Oblefias ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Maricor N. Soriano ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Caesar A. Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We developed a spectral imaging microscope that relies only on emission signals with no additional optics added to the microscope. Thus, it can resolve relative concentration in samples with dimensions below the diffraction limit and there is no need to see the actual structure of a sample to determine its composition as long as it emits light. From image colour we estimate the spectral emission of the sample. From the estimated fluorescence emission of multiple overlapping microscopic species, we demonstrate the microscope's spectral unmixing performance on light emitting diodes (LEDs), co-localized multi-color single-stained fluorescent microspheres, and multistained chromosomes and generate their two dimensional concentration maps.

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Issue

Article ID

SPP-2007-3C-03

Section

Instrumentation and Environmental Physics

Published

2007-10-24

How to Cite

[1]
WR Oblefias, MN Soriano, and CA Saloma, Two dimensional concentration maps through spectral estimation and spectral unmixing, Proceedings of the Samahang Pisika ng Pilipinas 25, SPP-2007-3C-03 (2007). URL: https://proceedings.spp-online.org/article/view/SPP-2007-3C-03.