Optical trapping and surgery of yeast cells cultured in liquid media
Abstract
We present optical trapping and surgery of living yeast cells cultured in viscous liquid media using near-infrared (NIR) ultrashort pulsed laser. Living yeast cells were exposed to continuous wave (CW) and femtosecond-pulsed focused laser of 780 nm wavelength at 20–60 mW of laser power. We show that focused laser beam operating in CW mode power does not affect the cell walls and can even be used for non-invasive optical trapping of yeast cells. Movement of trapped yeast cell is observed when the laser focus is scanned along a line. Furthermore, by changing the operation of the laser to femtosecond pulsed mode, we show that using a tightly focused laser dissected the rigid yeast cell walls resulting in intracellular organelles efflux.
Downloads
Published
Issue
Section
License
By submitting their manuscript to the Samahang Pisika ng Pilipinas (SPP) for consideration, the Authors warrant that their work is original, does not infringe on existing copyrights, and is not under active consideration for publication elsewhere.
Upon acceptance of their manuscript, the Authors further agree to grant SPP the non-exclusive, worldwide, and royalty-free rights to record, edit, copy, reproduce, publish, distribute, and use all or part of the manuscript for any purpose, in any media now existing or developed in the future, either individually or as part of a collection.
All other associated economic and moral rights as granted by the Intellectual Property Code of the Philippines are maintained by the Authors.
