3-dimensional direct observation of single-walled carbon nanotubes distribution in living cells

Authors

Serafin Delica ⋅ JP Department of Applied Physics, Osaka University
Katsumasa Fujita ⋅ JP Department of Applied Physics, Osaka University
Satoru Shoji ⋅ JP Department of Applied Physics, Osaka University
Yasushi Inoue ⋅ JP Department of Applied Physics, Osaka University
Satoshi Kawata ⋅ JP Department of Applied Physics, Osaka University

Abstract

We present the direct observation in three dimensions (3D) of single-walled carbon nanotubes (SWNT) in living cells via confocal microraman imaging. In contrast to current method which attaches fluorescent molecules to the SWNT, we have utilized the strong Raman signal coming the nanotubes to observe the SWNT distribution in a living cell by a confocal microscope. SWNTs functionalized by 1% F127 are able to penetrate the cell membrane but not the nuclear membrane. The SWNTs stays in the cytoplasm with no preferential distribution. Clustering is seen at low concentrations while an almost uniform distribution is observed for prolonged culturing time.

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Issue

2006: Proceedings of the 24th Samahang Pisika ng Pilipinas National Physics Congress

Article ID

SPP-2006-1G-02

Section

Instrumentation and Optics

Published

2006-10-25

How to Cite

[1]

S Delica, K Fujita, S Shoji, Y Inoue, and S Kawata, 3-dimensional direct observation of single-walled carbon nanotubes distribution in living cells, Proceedings of the Samahang Pisika ng Pilipinas 24, SPP-2006-1G-02 (2006). URL: https://proceedings.spp-online.org/article/view/SPP-2006-1G-02.