Sterilization of Bacillus subtilis-infected stainless steel using low-pressure H2O2 glow discharge plasma
Abstract
Sterilization of Bacillus subtilis-contaminated stainless steel dishes via a low-pressure DC glow discharge was investigated using 3% hydrogen peroxide (H2O2) and its mixture with argon (Ar). Total inactivation of 107 Bacillus cells was achieved in just 12 minutes using H2O2 plasma utilizing ∼10 W power and having sample temperature not exceeding 65°C. The fast sterilization process, under low input power and temperature, through the detoxifying nature of the plasma coupled with the chemical effect of the H2O2 makes the discharge facility commercially competitive.
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