Effect of noise and number of dyes in estimating fractional abundance in unmixing of composite fluorescence spectra

Authors

Wilma R. Oblefias ⋅ PH National Institute of Physics, University of the Philippines Diliman
Maricor N. Soriano ⋅ PH National Institute of Physics, University of the Philippines Diliman
Caesar A. Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We report the accuracy of a spectral unmixing algorithm as a function of noise and number of fluorescent probes. The algorithm is based on least-squares minimization with coefficient optimization and is applied to determine the relative fractional abundance of multiple fluorescent dyes from a composite spectrum. Simulation shows that accuracy increases with decreasing noise level and number of fluorescent dyes. An experimental result with 2 to 8% error on three co-localized fluorescent microspheres is presented using the developed approach.

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Issue

2005: Proceedings of the 23rd Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2005-1D-02

Section

Image and Signal Processing

Published

2005-10-26

How to Cite

[1]

WR Oblefias, MN Soriano, and CA Saloma, Effect of noise and number of dyes in estimating fractional abundance in unmixing of composite fluorescence spectra, Proceedings of the Samahang Pisika ng Pilipinas 23, SPP-2005-1D-02 (2005). URL: https://proceedings.spp-online.org/article/view/SPP-2005-1D-02.