Probing the attoliter focal volume of a high numerical aperture objective lens

Authors

Carlo Mar Blanca ⋅ PH National Institute of Physics, University of the Philippines Diliman
Edward Carlo Samson ⋅ PH National Institute of Physics, University of the Philippines Diliman
Bernardino Buenaobra ⋅ PH National Institute of Physics, University of the Philippines Diliman
Caesar Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We demonstrate experimentally the possibility of probing the tight confocal volume of a high numerical aperture (NA = 1.3) objective lens using mirror samples and mounted gold nanobeads. This has allowed us to measure axial resolution down to 450 nm fullwidth-half-maximum (FWHM) which corresponds well to the theoretical value of 420 nm derived using vector theory. The developed protocol is a significant tool for engineering point spread functions and compensating aberrations in confocal imaging systems.

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Issue

2003: Proceedings of the 21st Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2003-PS-03

Section

Plenary Sessions

Published

2003-10-22

How to Cite

[1]

CM Blanca, EC Samson, B Buenaobra, and C Saloma, Probing the attoliter focal volume of a high numerical aperture objective lens, Proceedings of the Samahang Pisika ng Pilipinas 21, SPP-2003-PS-03 (2003). URL: https://proceedings.spp-online.org/article/view/SPP-2003-PS-03.