Probing the attoliter focal volume of a high numerical aperture objective lens

Authors

  • Carlo Mar Blanca ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Edward Carlo Samson ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Bernardino Buenaobra ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Caesar Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We demonstrate experimentally the possibility of probing the tight confocal volume of a high numerical aperture (NA = 1.3) objective lens using mirror samples and mounted gold nanobeads. This has allowed us to measure axial resolution down to 450 nm fullwidth-half-maximum (FWHM) which corresponds well to the theoretical value of 420 nm derived using vector theory. The developed protocol is a significant tool for engineering point spread functions and compensating aberrations in confocal imaging systems.

SPP 2003 Proceedings Cover

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Published

2003-10-22

Issue

2003: Proceedings of the 21st Samahang Pisika ng Pilipinas Physics Congress

Section

Plenary Sessions

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How to Cite

[1]
“Probing the attoliter focal volume of a high numerical aperture objective lens”, Proc. SPP, vol. 21, no. 1, p. SPP-2003-PS-03, Oct. 2003, Accessed: Apr. 02, 2026. [Online]. Available: https://proceedings.spp-online.org/article/view/SPP-2003-PS-03