Probing the attoliter focal volume of a high numerical aperture objective lens

Authors

Carlo Mar Blanca National Institute of Physics, University of the Philippines Diliman
Edward Carlo Samson National Institute of Physics, University of the Philippines Diliman
Bernardino Buenaobra National Institute of Physics, University of the Philippines Diliman
Caesar Saloma National Institute of Physics, University of the Philippines Diliman

Abstract

We demonstrate experimentally the possibility of probing the tight confocal volume of a high numerical aperture (NA = 1.3) objective lens using mirror samples and mounted gold nanobeads. This has allowed us to measure axial resolution down to 450 nm fullwidth-half-maximum (FWHM) which corresponds well to the theoretical value of 420 nm derived using vector theory. The developed protocol is a significant tool for engineering point spread functions and compensating aberrations in confocal imaging systems.

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Issue

2003: Proceedings of the 21st Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2003-PS-03

Section

Plenary Sessions

Published

2003-10-22

How to Cite

[1]

CM Blanca, EC Samson, B Buenaobra, and C Saloma, Probing the attoliter focal volume of a high numerical aperture objective lens, Proceedings of the Samahang Pisika ng Pilipinas 21, SPP-2003-PS-03 (2003). URL: https://proceedings.spp-online.org/article/view/SPP-2003-PS-03.