Digital holographic microscopy

Authors

Selnalyn C. Ledesma National Institute of Physics, University of the Philippines Diliman
Marilou M. Cadatal National Institute of Physics, University of the Philippines Diliman
Percival F. Almoro National Institute of Physics, University of the Philippines Diliman

Abstract

Digital holography is implemented using a microscope objective (N.A., 0.35) for three-dimensional imaging reconstruction. The technique is applied in the investigation of a biological sample and particle size measurement. The samples used are a section of Pediculus humanus capitis (human head louse) mounted on a glass slide and microscopic glass beads used for sandblasting. Out-of-focus planes are refocused by digital holographic computations without the need for mechanical focusing. Size measurement is calibrated against the holographic reconstruction of a USAF negative resolution target recorded using the same objective.
An image deblurring algorithm based on inverse filtering is developed and implemented using the impulse response of the holographic system. Application of image deblurring results in sharper images.

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Issue

2003: Proceedings of the 21st Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2003-PB-18

Section

Poster Session PB

Published

2003-10-22

How to Cite

[1]

SC Ledesma, MM Cadatal, and PF Almoro, Digital holographic microscopy, Proceedings of the Samahang Pisika ng Pilipinas 21, SPP-2003-PB-18 (2003). URL: https://proceedings.spp-online.org/article/view/SPP-2003-PB-18.