Digital holographic microscopy
Abstract
Digital holography is implemented using a microscope objective (N.A., 0.35) for three-dimensional imaging reconstruction. The technique is applied in the investigation of a biological sample and particle size measurement. The samples used are a section of Pediculus humanus capitis (human head louse) mounted on a glass slide and microscopic glass beads used for sandblasting. Out-of-focus planes are refocused by digital holographic computations without the need for mechanical focusing. Size measurement is calibrated against the holographic reconstruction of a USAF negative resolution target recorded using the same objective.
An image deblurring algorithm based on inverse filtering is developed and implemented using the impulse response of the holographic system. Application of image deblurring results in sharper images.
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Creating essential bridges uniting physics, society and industry
22-25 October 2003, University of San Carlos, Cebu City