3D biological imaging using laser-scanning confocal fluorescence microscopy

Authors

Godofredo S. Bautista Jr. ⋅ PH National Institute of Physics, University of the Philippines Diliman
Carlo Mar Y. Blanca ⋅ PH National Institute of Physics, University of the Philippines Diliman
Caesar A. Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

In this work, the three-dimensional (3D) imaging capability of an inexpensive custom-built laser-scanning confocal fluorescence microscope (LSCFM) is demonstrated. Axial resolution is quantified by monitoring the response of the LSCFM to a fluorescence sea and an ultrathin fluorescence monolayer. Correspondingly, we present images of an autofluorescent pollen grain taken at different depths. The 3D volume-rendered image of the specimen is generated from the series of optical sections obtained. Results indicate unique morphology of the optically sectioned biological sample which are not evident in the individual optical sections.

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Issue

2003: Proceedings of the 21st Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2003-2E-05

Section

Biological and Medical Physics

Published

2003-10-22

How to Cite

[1]

GS Bautista, CMY Blanca, and CA Saloma, 3D biological imaging using laser-scanning confocal fluorescence microscopy, Proceedings of the Samahang Pisika ng Pilipinas 21, SPP-2003-2E-05 (2003). URL: https://proceedings.spp-online.org/article/view/SPP-2003-2E-05.