Spectral microscopy of bioluminescent and fluorescent samples using colored image

Abstract

A spectral microscope is utilized to determine the emission spectra of the following biological samples: 1) copepod (Arthropoda crustacea), 2) firefly (Photinus sp.) and 3) two types of sea anemone (Cnidaria anthozoa). We show that by adding synthetic emission spectra to even the distribution of peaks in the visible range of the ensemble for the calculation of basis spectra significantly improves the estimated spectral emission. Using only three to four colored images, fidelity, structural content and correlation quality of predicted spectra is almost equal to one. Minimum negativity constraint was not applied on the data because its effect is negligible and estimation is good even without it.