Determination of the lateral diffusion coefficient of lecithin-water systems using fluorescence spectroscopy
Abstract
The lateral diffusion coefficients, Ddiff, of phospholipid molecules in lecithin-water systems have been investigated. The method used for the quantitative determination is a spectroscopic technique. 1-pyrene dodecanoic acid was used as the spectroscopic probe. From the fluorescence spectra acquired, the monomer and excimer intensities were determined and were use to get Ddiff. The computed Ddiff are in the order of 10-8 suggesting that the systems observed are in the liquid crystalline Lα phase.