Hydrogen Raman shifter: A promising light source for two-color (two-photon) excitation fluorescence

Authors

Jonathan A. Palero ⋅ PH National Institute of Physics, University of the Philippines Diliman
Wilson O. Garcia ⋅ PH National Institute of Physics, University of the Philippines Diliman
Caesar A. Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

A Nd:YAG-pumped hydrogen Raman shifter is used as a light source for two-color (two-photon) excitation fluorescence. Coumarin 6H dye sample (peak absorption ≈ 394 nm, peak fluorescence ≈ 490 nm) is excited using the first two Stokes outputs (683 nm, 954 nm, two-color excitation = 398 nm) of a Raman shifter pumped by a 6.5 ns pulsed 532 nm-Nd:YAG laser (repetition rate = 10 Hz). Two-color fluorescence is generated even with focusing objectives of low numerical apertures (NA < 0.4). We observed the linear dependence of the two-color fluorescence signal with the product of the average intensities of the two Stokes excitation beams. The two-color fluorescence distribution is strongly localized around the common focus of the confocal excitation beams.

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Issue

2002: Proceedings of the 20th Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2002-3E-03

Section

Plenary Sessions

Published

2002-10-23

How to Cite

[1]

JA Palero, WO Garcia, and CA Saloma, Hydrogen Raman shifter: A promising light source for two-color (two-photon) excitation fluorescence, Proceedings of the Samahang Pisika ng Pilipinas 20, SPP-2002-3E-03 (2002). URL: https://proceedings.spp-online.org/article/view/SPP-2002-3E-03.