Hydrogen Raman shifter: A promising light source for two-color (two-photon) excitation fluorescence

Authors

Jonathan A. Palero National Institute of Physics, University of the Philippines Diliman
Wilson O. Garcia National Institute of Physics, University of the Philippines Diliman
Caesar A. Saloma National Institute of Physics, University of the Philippines Diliman

Abstract

A Nd:YAG-pumped hydrogen Raman shifter is used as a light source for two-color (two-photon) excitation fluorescence. Coumarin 6H dye sample (peak absorption ≈ 394 nm, peak fluorescence ≈ 490 nm) is excited using the first two Stokes outputs (683 nm, 954 nm, two-color excitation = 398 nm) of a Raman shifter pumped by a 6.5 ns pulsed 532 nm-Nd:YAG laser (repetition rate = 10 Hz). Two-color fluorescence is generated even with focusing objectives of low numerical apertures (NA < 0.4). We observed the linear dependence of the two-color fluorescence signal with the product of the average intensities of the two Stokes excitation beams. The two-color fluorescence distribution is strongly localized around the common focus of the confocal excitation beams.

Downloads

Issue

2002: Proceedings of the 20th Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2002-3E-03

Section

Plenary Sessions

Published

2002-10-23

How to Cite

[1]

JA Palero, WO Garcia, and CA Saloma, Hydrogen Raman shifter: A promising light source for two-color (two-photon) excitation fluorescence, Proceedings of the Samahang Pisika ng Pilipinas 20, SPP-2002-3E-03 (2002). URL: https://proceedings.spp-online.org/article/view/SPP-2002-3E-03.