Automated chromosome counting with color and grayscale microscope images

Authors

Ranzivelle Marianne L. Roxas ⋅ PH National Institute of Physics, University of the Philippines Diliman
Patricia Arielle A. Castro ⋅ PH National Institute of Physics, University of the Philippines Diliman
Maricor N. Soriano ⋅ PH National Institute of Physics, University of the Philippines Diliman
Caesar A. Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We present a system that can automatically count the number of chromosomes present in a metaphase spread. Image processing techniques on huesaturation-value (HSV) and normalized RG (rgI) color spaces and grayscale images are utilized in the detection of each chromosome. Upon comparing the performance of each technique, it is shown that operating in grayscale gives the best results. Testing the system with nine chromosome metaphase spread samples, an average success rate of 94% for grayscale images, 89% for HSV, and 93% for rgI is obtained.

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Issue

2002: Proceedings of the 20th Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2002-2B-06

Section

Biological and Medical Physics

Published

2002-10-23

How to Cite

[1]

RML Roxas, PAA Castro, MN Soriano, and CA Saloma, Automated chromosome counting with color and grayscale microscope images, Proceedings of the Samahang Pisika ng Pilipinas 20, SPP-2002-2B-06 (2002). URL: https://proceedings.spp-online.org/article/view/SPP-2002-2B-06.