Imaging properties of two-photon fluorescence excitation using nanosecond-pulse laser with tuning range from 450 to 1800 nm

Abstract

We present a study on two-photon fluorescence imaging properties using a light source capable of generating nanosecond light pulses with a wavelength tuning range of 450 to 1800 nm. The sample is rhodamine 6G dye solution in a quartz cuvette. Spectroscopic measurements of single-photon and twophoton excitation are made. The spatial characteristics of the fluorescence emission of the dye as function of exattion power are observed. In accordance with the two-photon fluorescence excitation phenomenon, the fluorescence intensity is measured to increase quadratically with the excitation power.