Imaging properties of two-photon fluorescence excitation using nanosecond-pulse laser with tuning range from 450 to 1800 nm

Authors

Vincent Daria ⋅ PH National Institute of Physics, University of the Philippines Diliman and Department of Applied Physics, Osaka University
Jose Omar Amistoso ⋅ PH National Institute of Physics, University of the Philippines Diliman
Wilson Garcia ⋅ PH National Institute of Physics, University of the Philippines Diliman
Caesar Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We present a study on two-photon fluorescence imaging properties using a light source capable of generating nanosecond light pulses with a wavelength tuning range of 450 to 1800 nm. The sample is rhodamine 6G dye solution in a quartz cuvette. Spectroscopic measurements of single-photon and twophoton excitation are made. The spatial characteristics of the fluorescence emission of the dye as function of exattion power are observed. In accordance with the two-photon fluorescence excitation phenomenon, the fluorescence intensity is measured to increase quadratically with the excitation power.

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Issue

1998: Proceedings of the 16th National Physics Congress of the Samahang Pisika ng Pilipinas

Article ID

SPP-1998-OP-10

Section

Optics and Photonics

Published

1998-10-27

How to Cite

[1]

V Daria, JO Amistoso, W Garcia, and C Saloma, Imaging properties of two-photon fluorescence excitation using nanosecond-pulse laser with tuning range from 450 to 1800 nm, Proceedings of the Samahang Pisika ng Pilipinas 16, SPP-1998-OP-10 (1998). URL: https://proceedings.spp-online.org/article/view/SPP-1998-OP-10.