SNR compensation for 3D imaging of turbid biological samples by two-photon fluorescence excitation

Authors

Vincent Ricardo Daria National Institute of Physics, University of the Philippines Diliman
Cynthia Palmes-Saloma Institute of Cell and Molecular Biology, Osaka University
Osamu Nakamura Department of Applied Physics, Osaka University
Satoshi Kawata Department of Applied Physics, Osaka University
Caesar Saloma National Institute of Physics, University of the Philippines Diliman

Abstract

We present a pre-detection technique for compensating the loss of detected fluorescence signal in two-photon fluorescence (TPF) imaging through an optically-thick biological sample. The signal-to-noise ratio (SNR) of the fluorescence image from deeper portions of the sample decreases due to multiple scattering caused by the inhomogeneities along the propagation paths. By modulating the average input power of a near-infrared mode-locked laser, the quadratic increase of the localized two-photon fluorescence intensity with respect to the excitation intensity is utilized to maintain the SNR of the axial images at different focal plane positions.

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Issue

1997: Proceedings of the 15th National Physics Congress of the Samahang Pisika ng Pilipinas

Article ID

SPP-1997-OP-17

Section

Optics and Photonics

Published

1997-10-27

How to Cite

[1]

VR Daria, C Palmes-Saloma, O Nakamura, S Kawata, and C Saloma, SNR compensation for 3D imaging of turbid biological samples by two-photon fluorescence excitation, Proceedings of the Samahang Pisika ng Pilipinas 15, SPP-1997-OP-17 (1997). URL: https://proceedings.spp-online.org/article/view/SPP-1997-OP-17.