lmaging properties of a Raman confocal-theta microscope

Authors

  • Ma. Obiminda Cambaliza ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Caesar Saloma ⋅ PH National Institute of Physics, University of the Philippines Diliman

Abstract

We compute the three-dimensional point spread function (PSF) of the Raman confocal-theta microscope that uses a grating spectrometer to determine the Raman spectra of a microscopic sample volume. In assessing the performance of the Raman confocal-theta microscope as a technique for materials analysis, it is necessary to investigate the PSF behavior as a function of the angle between the illumination and detection axes. The confocal-theta imaging configuration is important in cytometry, fluorescence microscopy, Raman spectroscopy and luminescence microscopy where the removal of the incident beam from the line of detection is imperative.

Downloads

Published

1997-10-27

How to Cite

[1]
MO Cambaliza and C Saloma, lmaging properties of a Raman confocal-theta microscope, Proceedings of the Samahang Pisika ng Pilipinas 15, SPP-1997-OP-03 (1997). URL: https://proceedings.spp-online.org/article/view/SPP-1997-OP-03.