Calibration curve for sugar concentration determination an LED-based spectrometer
Abstract
An LED-based spectrometer was developed using off-the-shelf light emitting diodes (LEDs) functioning both as the emitter and the detector. Its reliability was tested through a phenol-sulfuric acid assay to determine the concentration of sugars in solutions. From the transmittance values read using the LED-based spectrometer, a calibration curve was constructed. The development of a low-cost LED-based spectrometer can be useful for classroom experiments and scientific research since commercial spectrometers are expensive.