Imaging and orientational properties of single molecule detected by a high NA objective lens

Authors

Lean Dasallas ⋅ PH National Institute of Physics, University of the Philippines Diliman
Rafael Jaculbia ⋅ PH National Institute of Physics, University of the Philippines Diliman
Maria Vanessa Balois ⋅ JP Nearfield NanoPhotonics Research Team, RIKEN
Wilson Garcia ⋅ PH National Institute of Physics, University of the Philippines Diliman
Norihiko Hayazawa ⋅ JP Nearfield NanoPhotonics Research Team, RIKEN

Abstract

The ability of a high numerical aperture objective lens to detect single molecule emission as a function of molecule’s orientation has been determined numerically and is used to determine the emission image profile of single molecules excited by linearly polarized light. Several mechanism of illumination corresponding to different experimental configurations has been investigated and the effect of an interface in the resulting electric field distribution has been determined. The capacity of a high NA objective lens to collect the fluorescence signal has been determined and has been used to obtain the emission image profile of 1D dipole in full and annular illumination configuration. Result showed the potential use of emission image profile analysis as a tool to obtain 3D orientation of a single molecule.

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Issue

2015: Proceedings of the 33rd Samahang Pisika ng Pilipinas Physics Congress

Article ID

SPP-2015-1A-03

Section

Photonics and Terahertz, Optics and Signal Processing

Published

2015-06-03

How to Cite

[1]

L Dasallas, R Jaculbia, MV Balois, W Garcia, and N Hayazawa, Imaging and orientational properties of single molecule detected by a high NA objective lens, Proceedings of the Samahang Pisika ng Pilipinas 33, SPP-2015-1A-03 (2015). URL: https://proceedings.spp-online.org/article/view/1124.